Monoclonal Antibody (Rabbit) to Human DNA Methyltransferase 3a1 (DNMT-3a1)
Catalog Number: MAB-3a1
DNA methylation at CpG sites is an essential feature of gene regulation in higher eukarya [1-3]. Methylation of cytosine residues is correlated with silenced chromatin and there is strong evidence that inappropriate methylation can disrupt normal growth control circuits . The process is mediated by enzymes called DNA methyltransferases (DNMTs) and a number of different DNMT isoforms have been identified, cloned and characterized . DNMT isoforms fall into two broad groupings: 1) Maintenance activities which preferentially act on hemi-methylated DNA, and 2) De novo activities which initiate methylation on a unmethylated DNA targets. DNMT1 is a maintenance enzyme that most likely targets replicative or nascent DNA. DNMT3 type isoforms appear to be de novo methyltransferases. Other DNMTs are now known to be active but have not been classified (DNMT1b, DNMT2) while still other isoforms appear to possess key motifs of DNMT but lack sequences necessary for catalytic activity (DNMT3L). DNMT2 lacks the N-terminal regulatory domain found in DNMT1 and 3 but has a well conserved and intact catalytic domain. Earlier studies suggested that DNMT2 may not be an active methylase ; however, more recent data demonstrate DNMT2 activity in vivo using the ICM assay .
Anti-DNMT2 rabbit antibody was prepared using a peptide corresponding to residues mapping to the carboxyl terminus. Each vial contains 50 units of antibody at a concentration of 1 unit/ul. One unit corresponds to a 1:1000 dilution of stock antibody to give a working probe solution suitable for Western blotting. For example, a 1 unit/ul stock should be diluted 1:1000 to give 1 ml of probe solution and and 1 ul of a 2.5 units/ul would make 25 ml of working probe.
This antibody has been tested for use in Western blotting applications to detect the human DNMT2 and is suitable for testing DNMT2/DNA complex formation using the ICM assay described by Liu et al. . Reactivity to other species has not be examined, although DNMT2 is thought to be extremely well conserved. The antibody may be suitable for immunoprecipitation and immunohistochemistry; however, conditions optimal for these applications will have to be tested by the end user and may require more concentrated solutions than those recommended for Western blotting experiments.
Store the stock antibody at -20oC for long term storage or 4oC for short term storage. The antibody is stable for 6 months from date of shipping.
This product is designed for research use only and not suitable nor approved for diagnostic procedures in animals or humans.
1. Robertson, K. D. (2001) Oncogene 20, 3139-355.
2. Costello, J. F., Plass , C. (2001) J Med Genet 38, 285-303.
3. Baylin, S., et al. (2001) Human Molecular Genetics 10, 687-692.
4. Liu et al., (2003) Molec. Cell. Biol. 23: 2709-2719.
5. Okano, M. et al. (1998) Nucleic Acid. Res.26:2536-2540
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