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5-aza-2’-cytidine

Catalog Numbers and size:   

AZC-1 0.1 ml aqueous @ 10 mM

AZC-5 0.5 ml aqueous @ 10 mM

AZC-C Custom formulation (please inquire for details on packaging and pricing)

 

Introduction

DNA methylation at CpG sites is an essential feature of gene regulation in higher eukarya [1-3]. Methylation of cytosine residues is correlated with silenced chromatin and there is strong evidence that inappropriate methylation can disrupt normal growth control circuits [2]. The process is mediated by enzymes called DNA methyltransferases (DNMTs) and a number of different DNMT isoforms have been identified, cloned and characterized [1]. DNMT isoforms fall into two broad groupings: 1) Maintenance activities which preferentially act on hemi-methylated DNA, and 2) De novo activities which initiate methylation on a unmethylated DNA targets. Hypomethylating drugs, such as aza-deoxycytidine and aza-cytidine act by causing a covalent complex between active DNMT isoforms and DNA thereby depleting the cell of methylase activity. In addition to therapeutic potential in treating cancer, these drugs have clear value as research tools. The formation of covalent DNMT/DNA complexes allows researchers to ask key questions about the in vivo behavior of methylase isoforms. Methylation, Ltd. offers different hypomethylating drugs for investigative use in research and development. These agents have been pre-tested and are certified to work as hypomethylating drugs and for in vivo complex assays using ICM (for research purposes only).

 

Names

  • 5-azacytidine
  • 5-Aza-2'-cytidine
  • 5AZ 
  • 4-amino-1-beta-D-ribofuranosyl-1,3,5-triazin-2(1H)-one;
  • 4-amino-1-beta-D-ribofuranosyl-s-triazin-2(1H)-one; Antibiotic u 18496;

 

Description/Chemical Data

Molecular Formula: C8H12N4O5

Molecular Weight: 244

 

Storage

Store at -20oC (stable for 3 months from date of shipping).

 

Application:

This product is designed for research use only and not suitable nor approved for diagnostic procedures in animals or humans.

 

References

1. Robertson, K. D. (2001) Oncogene 20, 3139-355.

2. Costello, J. F., Plass , Christoph (2001) J Med Genet 38, 285-303.

3. Baylin, S., Esteller, M, Rountree, M, Bachman, K. Schuebel and Herman, G. (2001) Human Molecular Genetics 10, 687-692.

4. Liu et al., (2003) Molec. Cell. Biol. 23: 2709-2719.

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